International Journal of Advanced and Applied Sciences
Int. j. adv. appl. sci.
Print ISSN: 2313-626X
Volume 4, Issue 3 (March 2017), Pages: 51-58
Title: Preparation and characterization of cellulose and microcrystalline cellulose isolated from waste Leucaena leucocephala seeds
Author(s): Maryam Husin 1,2, Abd Rashid Li 3, Norfadhilah Ramli 2, Ahmad Zafir Romli 4, Mohd Idham Hakimi 2, Zul Ilham 2, *
1Faculty of Applied Science, Universiti Teknologi MARA, 44050 Shah Alam, Selangor, Malaysia
2Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
3Phytochemistry Program, Natural Products Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia
4Institute of Science, Universiti Teknologi MARA, 44050 Shah Alam Selangor, Malaysia
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Cellulose and microcrystalline cellulose (MCC) were isolated from waste Leucaena leucocephala seeds (LLS) by using acid hydrolysis method. Waste LLS are unused residues after extraction of oil for conversion to biodiesel. Cellulose from LLS (LLS-cellulose) has been isolated by using 80% acetic acid and 65% nitric acid to yield 33% cellulose. MCC was further prepared from the LLS-cellulose via the acid hydrolysis method and yield 71%. The obtained LLS-cellulose and LLS-MCC samples were comparatively investigated by Fourier transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), X-ray diffraction (XRD) and Field Emission Scanning Electron Microscopy technique (FESEM). FTIR second derivative showed the presence of minor amounts of bound hemicellulose and relatively free of lignin compound. The crystallinity index of LLS-cellulose is higher than LLS-MCC, indicating higher crystallize size and thermal decomposition. FESEM image also showed that there is smooth surface of raw LLS after hot boiling extraction for 2 hours. Thus, this study revealed that the lignin and hemicelluloses can be removed efficiently by using hot water treatment. In addition, cellulose components produced from waste LLS could be used as precursors of other industrial applications.
© 2017 The Authors. Published by IASE.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords: Waste Leucaena leucocephala seeds, Agricultural residues, Cellulose, Lignocellulosic, Biomass
Article History: Received 3 November 2016, Received in revised form 6 January 2017, Accepted 8 January 2017
Digital Object Identifier:
Husin M, Li AR, Ramli N, Romli AZ, Hakimi MI, and Ilham Z (2017). Preparation and characterization of cellulose and microcrystalline cellulose isolated from waste Leucaena leucocephala seeds. International Journal of Advanced and Applied Sciences, 4(3): 51-58
|Ahmed ME and Abdelati KA (2009). Chemical composition and amino acids profile of Leucaena leucocephala seeds. International Journal of Poultry Science, 8(10): 966-970.
|Battista OA (1950). Hydrolysis and crystallization of cellulose. Industrial and Engineering Chemistry, 42(3): 502-507.
|Bian J, Peng F, Peng XP, Peng P, Xu F, and Sun RC (2012). Acetic acid enhanced purification of crude cellulose from sugarcane bagasse: Structural and morphological characterization. BioResources, 7(4): 4626-4639.
|Cherian BM, Leão AL, Souza SFD, Costa LMM, Olyveira GMD, Kottaisamy M, Nagarajan ER, and Thomas S (2011). Cellulose nanocomposites with nanofibres isolated from pineapple leaf fibers for medical applications. Carbohydrate Polymers, 86(4):1790–1798.
|Chirayil CJ, Joy J, Mathew L, Mozetic M, Koetz J, and Thomas S (2014). Isolation and characterization of cellulose nanofibrils from Helicteres isora plant. Industrial Crops and Products, 59: 27-34.
|Duarte GV, Ramarao BV, Amidon TE, and Ferreira PT (2011). Effect of hot water extraction on hardwood kraft pulp fibers (Acer saccharum, Sugar Maple). Industrial and Engineering Chemistry Research, 50(17): 9949-9959.
|Elanthikkal S, Gopalakrishnapanicker U, Varghese S, and Guthrie JT (2010). Cellulose microfibres produced from banana plant wastes: Isolation and characterization. Carbohydrate Polymers, 80(3): 852-859.
|El-Sakhawy M and Hassan ML (2007). Physical and mechanical properties of microcrystalline cellulose prepared from agricultural residues. Carbohydrate Polymers, 67(1): 1-10.
|Fahma F, Iwamoto S, Hori N, Iwata T, and Takemura A (2010). Isolation, preparation, and characterization of nanofibers from oil palm empty-fruit-bunch (OPEFB). Cellulose, 17(5): 977-985.
|Haafiz MKM, Eichhorn SJ, Hassan A, and Jawaid M (2013). Isolation and characterization of microcrystalline cellulose from oil palm biomass residue. Carbohydrate Polymers, 93(2): 628-634.
|He J, Tang Y, and Wang SY (2007). Differences in morphological characteristics of bamboo fibres and other natural cellulose fibres: Studies on x-ray diffraction, solid state 13C-CP/MAS NMR, and second derivative FTIR spectroscopy data. Iranian Polymer Journal, 16(12): 807-818.|
|Henrique MA, Silvério HA, Neto WPF, and Pasquini D (2013). Valorization of an agro-industrial waste, mango seed, by the extraction and characterization of its cellulose nanocrystals. Journal of Environmental Management, 121: 202-209.
|Honda S, Miyata N, and Iwahori K (2002). Recovery of biomass cellulose from waste sewage sludge. Journal of Material Cycles and Waste Management, 4(1): 46-50.|
|Ilindra A and Dhake JD (2008). Microcrystalline cellulose from bagasse and rice straw. Indian Journal of Chemical Technology, 15(5): 497-499.|
|Jahan MS, Saeed A, He Z, and Ni Y (2011). Jute as raw material for the preparation of microcrystalline cellulose. Cellulose, 18(2): 451-459.
|Jiang F and Hsieh YL (2015). Cellulose nanocrystal isolation from tomato peels and assembled nanofibers. Carbohydrate Polymers, 122: 60-68.
|Johar N, Ahmad I, and Dufresne A (2012). Extraction, preparation and characterization of cellulose fibres and nanocrystals from rice husk. Industrial Crops and Products, 37(1): 93-99.
|Kalita RD, Nath Y, Ochubiojo ME, and Buragohain AK (2013). Extraction and characterization of microcrystalline cellulose from fodder grass; Setaria glauca (L) P. Beauv, and its potential as a drug delivery vehicle for isoniazid, a first line antituberculosis drug. Colloids and Surfaces B: Biointerfaces, 108: 85-89.
|Lubes ZIZ and Zakaria M (2009). Analysis of parameters for fatty acid methyl esters production from refined palm oil for use as biodiesel in the single- and two-stage processes. Malaysian Journal of Biochemistry and Molecular Biology, 17(1): 5-9.|
|Mandal A and Chakrabarty D (2011). Isolation of nanocellulose from waste sugarcane bagasse (SCB) and its characterization. Carbohydrate Polymers, 86(3): 1291-1299.
|Merci A, Urbano A, Grossmann MVE, Tischer CA, and Mali S (2015). Properties of microcrystalline cellulose extracted from soybean hulls by reactive extrusion. Food Research International, 73: 38-43.
|Nazir MS, Wahjoedi BA, Yussof AW, and Abdullah MA (2013). Eco-friendly extraction and characterization of cellulose from oil palm empty fruit bunches. BioResources, 8(2): 2161-2172.
|Normand ML, Moriana R, and Ek M (2014). Isolation and characterization of cellulose nanocrystals from spruce bark in a biorefinery perspective. Carbohydrate Polymers, 111: 979-987.
|Nuruddin M, Chowdhury A, Haque SA, Rahman M, Farhad SF, Jahan MS, and Quaiyyum A (2011). Extraction and characterization of cellulose microfibrils from agricultural wastes in an integrated biorefinery initiative. Cellulose Chemistry Technology, 45(5-6): 347-354.|
|Pandey VC and Kumar A (2013). Leucaena leucocephala: an underutilized plant for pulp and paper production. Genetic Resources and Crop Evolution, 60(3): 1165-1171.
|Park S, Baker JO, Himmel ME, Parilla PA, and Johson DK (2010). Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance. Biotechnology for Biofuels, 3(1): 1-10.
|Phoo ZWMM, Ilham Z, Goembira F, Razon L, and Saka S (2012). Physico-chemical properties of biodiesel from various feedstocks. In: Yao T (Eds.), Green Energy and Technology: 113-121. Springer, Japan.|
|Poletto M, Júnior HLO, and Zattera AJ (2014). Native cellulose: Structure, characterization and thermal properties. Materials (Basel), 7(9): 6105-6119.
|Shankar S and Rhim JW (2016). Preparation of nanocellulose from micro-crystalline cellulose: The effect on the performance and properties of agar-based composite films. Carbohydrate Polymers, 135: 18-26.
|Soom RM, Aziz AA, Hassan WHW, and Top AGM (2009). Solid-state characteristics of microcrystalline cellulose from oil palm empty fruit bunch fibre. Journal of Oil Palm Research, 21: 613-620.|
|Sun JX, Sun XF, Zhao H, and Sun RC (2004). Isolation and characterization of cellulose from sugarcane bagasse. Polymer Degradation and Stability, 84(2): 331-339.
|Trache D, Donnot A, Khimeche K, Benelmir R, and Brosse N (2014). Physico-chemical properties and thermal stability of microcrystalline cellulose isolated from Alfa fibres. Carbohydrate Polymers, 104: 223-230.
|Wang D, Shang SB, Song ZQ, and Lee MK (2010). Evaluation of microcrystalline cellulose prepared from kenaf fibers. Journal of Industrial and Engineering Chemistry, 16(1): 152-156.
|Yang H, Yan R, Chen H, Lee DH, and Zheng C (2007). Characteristics of hemicellulose, cellulose and lignin pyrolysis. Fuel, 86(12): 1781-1788.