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EISSN: 2313-3724, Print ISSN:2313-626X

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Volume 4, Issue 11 (November 2017), Pages: 17-21


Original Research Paper

Title: Crude extract yield, total phenolics, and total flavonoids from the ink of sea hare (Dolabella auricularia)

Author(s): Janeth C. Tayone 1, *, Romeo M. Del Rosario 2


1Institute of Agriculture and Life Sciences, Davao Oriental State College of Science and Technology, City of Mati, 8200, Davao Oriental, Philippines
2Department of Chemistry, College of Science and Mathematics, University of Science and Technology of Southern Philippines, Lapasan, Cagayan de Oro, 9000, Philippines

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The crude extract yield, total phenolics and total flavonoids of the ethanol and water extracts of sea hare (Dolabella auricularia) were evaluated. The crude extract yield was determined gravimetrically while Folin-Ciocalteau and Aluminum chloride methods were used for the determination of the total phenolics and total flavonoids, respectively. The results showed that the crude extract yield for the ethanol fraction was higher (7.657 x 103 mg/ 100 mL of fresh ink sample) compared to water (2.937 x 103 mg/ 100 mL of fresh ink sample). The same pattern was also exhibited for both total phenolics and total flavonoids where a significant difference was observed at p<0.05. The total phenolics of the ethanol extract were higher at 2.77 mg gallic acid / mL compared to that of the water extract at only 0.75 mg gallic acid/ mL. While the total flavonoids of the ethanol extract was 32.75 mg quercetin/mL against that of the water extract which was only 9.42 mg quercetin/ mL. The results indicate that ethanol is a better solvent than water for the extraction of the phenolics, flavonoids and other components. Furthermore, the presence of phenolics and flavonoids in considerable quantity suggests that ink of sea hare is a potential source of bioactive compounds that have antioxidant activity which can be developed for nutritional or pharmacological use. 

© 2017 The Authors. Published by IASE.

This is an open access article under the CC BY-NC-ND license (

Keywords: Dolabella auricularia, Flavonoids, Phenolics, Pujada Bay, Sea hare

Article History: Received 23 June 2017, Received in revised form 27 August 2017, Accepted 13 September 2017

Digital Object Identifier:


Tayone JC and Rosario RMD (2017). Crude extract yield, total phenolics, and total flavonoids from the ink of sea hare (Dolabella auricularia). International Journal of Advanced and Applied Sciences, 4(11): 17-21

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References (17)

  1. Abirami P, Arumugam M, Ajithkumar TT, and Balasubramanian T (2011). Isolation and characterization of 37 kDa heparinase from the purple fluid of Dolabella auricularia. Indian Journal of Geo-Marine Siences, 40(1): 112-116. 
  2. Ardekani MRS, Hajimahmoodi M, Oveisi MR, Sadeghi N, Jannat B, Ranjbar AM, and Moridi T (2011). Comparative antioxidant activity and total flavonoid content of Persian pomegranate (Punica granatum L.) cultivars. Iranian Journal of Pharmaceutical Research: IJPR, 10(3): 519-524.     
  3. Cherif E, Lahbib K, Charaf M, and Yahaia MND (2015). Antioxidant activity of Aplysia depilans ink collected from Bizerte Channel (NE Tunisia). Algerian Journal of Natural Products, 3(1): 138-145.     
  4. Dhanani T, Shah S, Gajbhiye NA, and Kumar S (2017). Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arabian Journal of Chemistry, 10(1): S1193-S1199. 
  5. Jakopic J and Veberic R (2009). Extraction of phenolic compounds from green walnut fruits in different solvents. Acta Agriculturae Slovenica, 93(1): 11-15. 
  6. Jang HD, Chang KS, Huang YS, Hsu CL, Lee SH, and Su MS (2007). Principal phenolic phytochemicals and antioxidant activities of three Chinese medicinal plants. Food Chemistry, 103(3): 749-756. 
  7. Kalita P, Tapan BK, Pal TK, and Kalita R (2013). Estimation of total flavonoids content (TFC) and anti-oxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. Journal of Drug Delivery and Therapeutics, 3(4): 33-37. 
  8. Khatiwora E, Adsul VB, Kulkarni MM, Deshpande NR, and Kashalkar RV (2010). Spectroscopic determination of total phenol and flavonoid contents of Ipomoea carnea. International Journal of ChemTech Research, 2(3): 1698-1701.     
  9. Kicklighter CE and Derby CD (2006). Multiple components in ink of the sea hare Aplysia californica are aversive to the sea anemone Anthopleura sola. Journal of Experimental Marine Biology and Ecology, 334(2): 256-268. 
  10. Mamelona J, Pelletier É, Girard-Lalancette K, Legault J, Karboune S, and Kermasha S (2007). Quantification of phenolic contents and antioxidant capacity of Atlantic sea cucumber, Cucumaria frondosa. Food Chemistry, 104(3): 1040-1047. 
  11. Pennings SC, Paul VJ, Dunbar DC, Hamann MT, Lumbang WA, Novack B, and Jacobs RS (1999). Unpalatable compounds in the marine gastropod Dolabella auricularia: Distribution and effect of diet. Journal of Chemical Ecology, 25(4): 735-755. 
  12. Proksch P, Edrada R, and Ebel R (2002). Drugs from the seas-current status and microbiological implications. Applied Microbiology and Biotechnology, 59(2): 125-134.  PMid:12111137     
  13. Simmons TL, Andrianasolo E, McPhail K, Flatt P, and Gerwick WH (2005). Marine natural products as anticancer drugs. Molecular Cancer Therapeutics, 4(2): 333-342.  PMid:15713904     
  14. Sreejamole KL and Radhakrishnan CK (2010). Preliminary qualitative chemical evaluation of the extracts from mussel Perna viridis. International Journal of Pharmaceutical Sciences Review and Research, 5(2): 38-42.     
  15. Stanojević L, Stanković M, Nikolić V, Nikolić L, Ristić D, Čanadanovic-Brunet J, and Tumbas V (2009). Antioxidant activity and total phenolic and flavonoid contents of Hieracium pilosella L. extracts. Sensors, 9(7): 5702-5714.  PMid:22346723  PMCid:PMC3274148     
  16. Sultana B, Anwar F, and Ashraf M (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14(6): 2167-2180.  PMid:19553890 
  17. Varshney A and Singh V (2013). Effects of algal compounds on cancer cell line. Journal of Experimental Biology and Agricultural Sciences, 1(5): 337-352.