International Journal of

ADVANCED AND APPLIED SCIENCES

EISSN: 2313-3724, Print ISSN: 2313-626X

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 Volume 9, Issue 12 (December 2022), Pages: 77-84

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 Original Research Paper

 Influence of KCNQ1 and TCF7L2 genes associated with the role of type 2 diabetes

 Author(s): Nada Alqadri 1, Nuha A. Abdelmutalab 2, Sitalnesa Abdelhafeez 3, Atyah Y. Alzahrani 4, Omima Gadalla Mohamed 3, Aeshah Hassan 5, Omaima Nasir 1, *

 Affiliation(s):

 1Department of Biology, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
 2Department of Infection Control, King Faisal Medical Complex. P.O. Box 2265, Taif 21944, Saudi Arabia
 3Department of Communicable Diseases Control Directorate, Taif 21944, Saudi Arabia
 4Department of Family Medicine, Directorate, Taif 21944, Saudi Arabia
 5Department of Clinical Laboratory Sciences, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

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 * Corresponding Author. 

  Corresponding author's ORCID profile: https://orcid.org/0000-0002-3021-6473

 Digital Object Identifier: 

 https://doi.org/10.21833/ijaas.2022.12.010

 Abstract:

T2DM is a long-term metabolic disorder characterized by either the pancreas' inability to produce enough insulin or the body's inability to properly utilize the insulin it produces. The β-cell function and blood glucose homeostasis are two areas where TCF7L2 (Transcription factor 7 like 2) appears to be a significant candidate gene. KCNQ1 (potassium voltage-gated channel subfamily, member 1 has been discovered as a T2DM susceptibility gene in Asian populations by genome-wide association studies with rs2237892 polymorphism and an increased risk of developing T2DM. The aim of this study was to investigate the association between rs7903146 and rs2237892 SNP studies in T2DM patients. In this study, 60 T2DM cases and 60 controls were selected. Genotyping was performed for rs7903146 and rs2237892 SNPs using specific primers and restriction enzymes, then all PCR products were loaded on an agarose gel stained with ethidium bromide. The current study results confirmed rs7903146 SNP was strongly associated with genotype (OR-4.14; 95%CI:1.07-15.98; p=0.02) and allele frequencies (OR-4.60; 95%CI:1.66-12.70; p=0.001) whereas in rs2237892 SNP was not associated with any of the genotypes (OR-4.29; 95%CI:0.46-39.58; p=0.16; OR-3.21) or allele frequencies (OR-6.26; 95%CI:0.74-52.83; p=0.055). The current study results were found to be associated with global studies carried out in rs7903146 and rs2237892 SNP. The strength of this current study was to involve Saudi nationalities and we have screened rs7903146 and rs2237892 SNPs which plays a major role in T2DM. Involving 60 T2DM cases/60 controls was the major limitation of this study. Missing validation through Sanger sequencing analysis was one of the limitations of this study. In conclusion, the current study results confirmed rs7903146 SNP was strongly associated with T2DM and rs2237892 SNP was not associated with T2DM patients.

 © 2022 The Authors. Published by IASE.

 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 Keywords: Type 2 diabetes mellitus, Rs7903146, Rs2237892, TCF7L2, KCNQ1

 Article History: Received 12 April 2022, Received in revised form 4 August 2022, Accepted 29 August 2022

 Acknowledgment 

No Acknowledgment.

 Compliance with ethical standards

 IRB details: 

The ethical grant for this study was obtained from Institutional Review Board and a patient-informed consent form was obtained for all subjects who participated in this study. In this study, we have excluded the participants who weren’t signed the consent form. This study was conducted in accordance with the principles outlined in the Helsinki Declaration. This is a case-control study.

 Conflict of interest: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

 Citation:

 Alqadri N, Abdelmutalab NA, Abdelhafeez S, Alzahrani AY, Mohamed OG, Hassan A, and Nasir O (2022). Influence of KCNQ1 and TCF7L2 genes associated with the role of type 2 diabetes. International Journal of Advanced and Applied Sciences, 9(12): 77-84

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 Figures

 Fig. 1 Fig. 2 Fig. 3

 Tables

 Table 1 Table 2 Table 3 Table 4 

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