International journal of

ADVANCED AND APPLIED SCIENCES

EISSN: 2313-3724, Print ISSN:2313-626X

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 Volume 5, Issue 4 (April 2018), Pages: 30-41

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 Original Research Paper

 Title: Modulation of cancer cell proliferation by unusually produced β(1-6) linked Mannan-oligosaccharides and β(1-6) linked Galactooligosaccharides using β–galactosidase from Aspergillus oryzae

 Author(s): Roger Salvacion Tan 1, 2, *

 Affiliation(s):

 1Department of Chemistry, College of Science, De La Salle University, 2401 Taft Avenue, Manila 0922, Philippines
 2Department of Chemistry, College of Science and Mathematics, Mindanao State University-Iligan Institute of Technology, A. Bonifacio Avenue, TibangaIligan City 9200, Philippines

 https://doi.org/10.21833/ijaas.2018.04.005

 Full Text - PDF          XML

 Abstract:

This study introduced an alternative way to produce NDOs, utilizing the recently reported unusual β-galactosynthase-β-mannosynthase dual activity of this enzyme, using free monosaccharide substrates. The enzyme successfully converted galactose and mannose monomer sugars efficiently to NDOs. Glycoblotting and MALDI-TOFMS analyses confirmed that the enzyme produced a maximum of 46.37% galacto-oligosaccharides (36.88% galactobiose, 8.52 % galactotriose, and 0.9746 % galactotetraose) from galactose monosaccharides and 32.62 % mannan-oligosaccharides (30.95 % mannobiose and 1.68 % mannotriose) from mannose monosaccharides. Both MOS and GOS modulated cell growth of different human cancer cell lines, with apparent antiproliferative effect on prostate adenocarcinoma (PC-3) cells and mitogenic effects on A549, HepG2, and HT-29. Mitochondrial membrane potential and careful morphological evaluation of PC-3 cells treated with GOS and MOS suggested that MOS treated cells showed possible inhibition of cellular adhesion that might have caused apoptosis to advance.  The high-yielding NDOs synthase character of this cheaply available β–galactosidase makes this new strategy a good potential alternative in producing prebiotic NDOs. 

 © 2018 The Authors. Published by IASE.

 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 Keywords: Non-digestible oligosaccharides, Galacto-oligosaccharides, Mannan-oligosaccharides, Asprgillus oryzae, β-galactosidase, Glycoblotting

 Article History: Received 2 November 2017, Received in revised form 28 January 2018, Accepted 29 January 2018

 Digital Object Identifier: 

 https://doi.org/10.21833/ijaas.2018.04.005

 Citation:

 Tan RS (2018). Modulation of cancer cell proliferation by unusually produced β(1-6) linked Mannan-oligosaccharides and β(1-6) linked Galactooligosaccharides using β–galactosidase from Aspergillus oryzae. International Journal of Advanced and Applied Sciences, 5(4): 30-41

 Permanent Link:

 http://www.science-gate.com/IJAAS/2018/V5I4/Tan.html

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